14 June 2011 Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods
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Abstract
We report on the optoacoustic (OA) imaging of the whole mouse body using a biocompatible contrast agent - highly purified, pegylated gold nanorods (GNR) - which has strong optical absorption in the near-infrared region and low level of toxicity. In vitro toxicity studies showed no significant change in survival rates of the cultured normal epithelium IEC-6 cells when incubated for 24 hours with up to 1 nM of GNR. In vivo toxicity studies in nude mice showed no pathological changes in liver 1 month after the IV injection of GNR with intra-body concentration around 0.25-0.50 nM. In order to study the enhancement of the OA contrast and accumulation of GNR in different tissues, we performed 3D OA imaging of live nude mice with IV-injected GNR. The enhancement of the OA contrast in comparison with the images of the untreated mice was visible starting 1 hour after the GNR injection. The OA contrast of kidneys, liver, and spleen peaked at about 2-3 days after the administration of the GNR, and then was gradually reducing.
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Richard Su, Richard Su, Anton Liopo, Anton Liopo, Hans-Peter Brecht, Hans-Peter Brecht, Sergey Ermilov, Sergey Ermilov, Kirill Larin, Kirill Larin, Alexander A. Oraevsky, Alexander A. Oraevsky, } "Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods", Proc. SPIE 8089, Molecular Imaging III, 808902 (14 June 2011); doi: 10.1117/12.889757; https://doi.org/10.1117/12.889757
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