Scanning Optical Microscopy Applied To Fluorometry

Gerard Roblin; Leon Bernstein

doi:10.1117/12.941499

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3 August 1987 Scanning Optical Microscopy Applied To Fluorometry

Gerard Roblin, Leon Bernstein

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Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941499
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

Scanning Optical Microscopy, able to reconstruct, pixel after pixel, low noise images with the expected microscope resolution, is especially suitable for quantitative microscopy. Use of a bright, monochromatic spot of light extends its field of application to fluo-rescence Microscopy. Description of a typical device is given and the problems encountered to realize the scan of the laser beam are discussed. Results relating to transmitted light images as well as to epifluorescence images and spectral analysis are shown.

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Gerard Roblin and Leon Bernstein "Scanning Optical Microscopy Applied To Fluorometry", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941499

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