The detection and diagnosis of diseases have improved in recent years. Developments in diagnostic techniques
have helped to improve treatment in the early stages and to avoid many risks to patients. One such technique is
optical coherence tomography (OCT), which is used in many medical applications to perform internal
microstructural imaging of the human body at high resolution (typically 10 μm), at high speed and in real time.
OCT is non-invasive and can be used as a contact or non-contact technique to obtain an image. In medicine,
there are many applications that involve OCT, such as in ophthalmology, gastroenterology, cardiology and
oncology. This work demonstrates the use of an OCT system incorporating a swept laser source with a high
sweep rate of 16 kHz over a wide range of wavelengths (1260 nm to 1390 nm) to measure the thickness of the
peritoneal membrane in mice of different sizes and weights. The real axial line speed is limited by the source
that is used in the OCT system. The optical source has a bandwidth of ▵λ =110 nm, centred at λ0 =1325 nm. The
aim of this study is to investigate the thickening of the peritoneal membrane which can occur during prolonged
peritoneal dialysis in mice. As part of this preliminary study, healthy mice of different weights were euthanized
and the thickness of the peritoneal membrane was measured using OCT. The aim was to gather data on the
expected range of thicknesses present in healthy animals for future studies. For this work, two locations on the
peritoneal membrane of each of 20 mice were imaged.