2 June 2011 In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation
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Abstract
The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm², respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.
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Nora Bloise, Nora Bloise, Enrica Saino, Enrica Saino, Francesca Bragheri, Francesca Bragheri, Paolo Minzioni, Paolo Minzioni, Ilaria Cristiani, Ilaria Cristiani, Marcello Imbriani, Marcello Imbriani, Livia Visai, Livia Visai, } "In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation", Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80920U (2 June 2011); doi: 10.1117/12.889873; https://doi.org/10.1117/12.889873
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