Triple beam optical trap for microsyringe construction

William T. Ramsay; Muriel Bechu; Vladimir A. Bolanos Quinones; Yongfeng Mei; Oliver G. Schmidt; Lynn Paterson

doi:10.1117/12.893667

9 September 2011 Triple beam optical trap for microsyringe construction

William T. Ramsay, Muriel Bechu, Vladimir A. Bolanos Quinones, Yongfeng Mei, Oliver G. Schmidt, Lynn Paterson

Proceedings Volume 8097, Optical Trapping and Optical Micromanipulation VIII; 809708 (2011) https://doi.org/10.1117/12.893667
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

A limited range of instruments are available which allow the controlled injection of sub-picolitre volumes; microfluidic devices and commercially produced mechanical microinjection systems accounting for the majority. We present an optically controlled microsyringe capable of dispensing femtolitres of liquid. Triple beam optical tweezers are used to manipulate hollow glass microneedles and also polymer microspheres which were used as 'handles' to assist the manipulation of microneedles and 'plungers' to dispense liquid from the microneedle. Standard optical tweezers were used with the addition of a Ronchi ruling (250 lines per inch) mounted in the image relay telescope. The diffraction pattern generated by the Ronchi ruling produced three optical traps in the sample chamber. Trap spacing was controlled by translating the ruling along the axis of beam propagation within the image relay telescope. Utilizing the three-beam trap, it was possible to manipulate pulled, borosilicate capillaries (5-150μm in length, 1-10μm in diameter) both perpendicular and parallel to the axis of the capillary. Rolled SiO/SiO2 microtubes (4μm diameter, 50μm long) were also manipulated, however in this case polymer microspheres were used as 'handles'. In both cases the microneedles did not align vertically along the propagation axis; an advantage over using a single beam optical trap. Tweezing a microsphere within a microneedle dispenses femtolitres of liquid from the needle. The force exerted on microneedles is calculated to be in the order of picoNewtons so may have applications where femtolitre volumes must be controllably delivered beyond a barrier, such as single cell microinjection.

Citation Download Citation

William T. Ramsay, Muriel Bechu, Vladimir A. Bolanos Quinones, Yongfeng Mei, Oliver G. Schmidt, and Lynn Paterson "Triple beam optical trap for microsyringe construction", Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 809708 (9 September 2011); https://doi.org/10.1117/12.893667

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical tweezers

Optical spheres

Particles

Ronchi rulings

Capillaries

Telescopes

Relays

Show All Keywords

Keywords/Phrases

Search In:

Publication Years