9 September 2011 Laser microbeam - kinetic studies combined with molecule - structures reveal mechanisms of DNA repair
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Proceedings Volume 8097, Optical Trapping and Optical Micromanipulation VIII; 80970D (2011); doi: 10.1117/12.893652
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States
Abstract
Kinetic studies on double strand DNA damages induced by a laser microbeam have allowed a precise definition of the temporal order of recruitment of repair molecules. The order is KU70 / KU80 - XRCC4 --NBS1 -- RAD51. These kinetic studies are now complemented by studies on molecular structures of the repair proteins, using the program YASARA which does not only give molecular structures but also physicochemical details on forces involved in binding processes. It turns out that the earliest of these repair proteins, the KU70 / KU80 heterodimer, has a hole with high DNA affinity. The next molecule, XRCC4, has a body with two arms, the latter with extremely high DNA affinity at their ends and a binding site for Ligase 4. Together with the laser microbeam results this provides a detailed view on the early steps of DNA double strand break repair. The sequence of DNA repair events is presented as a movie.
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B. Altenberg, K. O. Greulich, "Laser microbeam - kinetic studies combined with molecule - structures reveal mechanisms of DNA repair", Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970D (9 September 2011); doi: 10.1117/12.893652; https://doi.org/10.1117/12.893652
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KEYWORDS
Molecules

Proteins

Molecular lasers

Temporal resolution

Performance modeling

Quantum cascade lasers

Visualization

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