The development of a sensing platform capable of detecting and identifying hazards including biological, chemical, and
energetic in nature is a long sought after goal of the Army and many other first responders. Surface enhanced Raman
scatting (SERS) is one spectroscopic technique gaining popularity as a solution to many sensing needs due to its many
advantages such as high sensitivity, little to no sample preparation required, and use in numerous environmental
settings). Despite all the advantages of SERS, it still remains a marginalized sensing technique primarily due to the
challenges in fabricating a reliable, highly sensitive and reproducible nanoscale surface. In this work, we show that
many of these challenges have been overcome with a newly developed commercially available Klarite SERS substrate. These substrates are fabricated in a fashion similar to standard Klarite substrates, but due to changes in size and spacing of the inverted pyramidal structurethere is an overall increase of SERS sensing capabilities of up to 4 orders of magnitude. In this proceeding paper, the next generation Klarite (308 and 309) substrates are characterized, analyte sensitivity demonstrated at 633 nm and 785 nm, and a brief discussion of their biological sensing capabilities is presented.