12 September 2011 Nanobarcoding: a novel method of single nanoparticle detection in cells and tissues for nanomedical biodistribution studies
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Abstract
Determination of whether nanoparticles accumulate in target or non-target tissues is critical in assessing a nanoparticle formulation for nanomedical purposes. There is an overwhelming need for a sensitive and efficient imaging-based method that can (1) detect small numbers of (even single) nanoparticles, (2) associate nanoparticle uptake with cell type, and (3) allow for rapid detection in large tissue samples. We propose a novel method for nanoparticle detection that utilizes an oligonucleotide "nanobarcode" conjugated to the nanoparticle surface, which amplifies the optical signal from a single nanoparticle via in situ PCR. Herein, we describe the design process of the nanobarcoding method.
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Trisha Eustaquio, Trisha Eustaquio, James F. Leary, James F. Leary, } "Nanobarcoding: a novel method of single nanoparticle detection in cells and tissues for nanomedical biodistribution studies", Proc. SPIE 8099, Biosensing and Nanomedicine IV, 80990V (12 September 2011); doi: 10.1117/12.893915; https://doi.org/10.1117/12.893915
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