16 September 2011 Measurement of stress and strain applied to electrochemically aligned collagen fibres by second-harmonic generation microscopy
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Abstract
In this study, we developed a novel strain measurement technique for electrochemically aligned collagen (ELAC) fibres using second harmonic generation (SHG) microscopy. The ELAC fibres were prepared by a typical electrochemical method and were subjected to cross-linking. For comparison with natural collagen fibres, polarization dependency of the prepared ELAC fibres and that of a human Achilles' tendon were evaluated. The results showed that, because of crosslinking, the ELAC fibres exhibit polarization dependency similar to that of the tendon but only in a region close to the tendon. The relationship between SHG and the applied strain was determined by a combination of SHG microscopy and tensile tests. The SHG from the ELAC fibres changed in the high strain region because of the applied stress.
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Nobutaka Goami, Keisuke Yoshiki, Takahiro Namazu, Shozo Inoue, "Measurement of stress and strain applied to electrochemically aligned collagen fibres by second-harmonic generation microscopy", Proc. SPIE 8113, Linear and Nonlinear Optics of Organic Materials XI, 81130E (16 September 2011); doi: 10.1117/12.894151; https://doi.org/10.1117/12.894151
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