7 September 2011 Advanced optical design for DNA sequencing systems
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Recent advances in DNA sequencing techniques require the development of high NA immersion microscope systems with large field-of-view, increased resolution and very tight distortion correction. This paper discusses the optical design and tolerance analysis of a NA 1.0 immersion microscope system with a field of view over 1 mm, which is to be used for four-color fluorescence spectroscopy. The microscope system includes an immersion objective lens and four tube lenses, and is corrected for a wide wavelength range from 490 nm to 712 nm. We will discuss critical aspects of designing this kind of optical system, including special glass selection required for chromatic correction.
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Kirill Sobolev, Kirill Sobolev, Adel Joobeur, Adel Joobeur, Craig Uhrich, Craig Uhrich, David Aziz, David Aziz, } "Advanced optical design for DNA sequencing systems", Proc. SPIE 8128, Current Developments in Lens Design and Optical Engineering XII; and Advances in Thin Film Coatings VII, 812809 (7 September 2011); doi: 10.1117/12.894268; https://doi.org/10.1117/12.894268


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