3-D Microscope Imaging By Optical 3-D CT

S. Minami; O. Nakamura; S. Kawata

doi:10.1117/12.967291

1 January 1987 3-D Microscope Imaging By Optical 3-D CT

S. Minami, O. Nakamura, S. Kawata

Proceedings Volume 0813, Optics and the Information Age; (1987) https://doi.org/10.1117/12.967291
Event: 14th Congress of the International Commission for Optics, 1987, Quebec, Canada

Abstract

The success of X-ray CT (Computed Tomography) in clinical medicine has motivated studies of nondestructive three-dimensional (3-D) observation of samples by light wave propagation through 3-D samples. The optical CT technique has been applied to 3-D measurements of refractive index, air pollutants, flow, plasma density, and birefringence. We have proposed a new method of optical 3-D CT for microscopic 3-D imaging with a conventional transmission-microscope optics and an advanced computer algorithm. By this method, inside 3-D structures of alive biological samples can be reconstructed without slicing-preparation of samples. In this paper, we describe the principle of the developed optical projeciton system with the analysis of its 3-D OTF, and the reconstruction algorithm with constraints. An experimental example of multispectral 3-D reconstruction is shown for a pollen of pine.

Citation Download Citation

S. Minami, O. Nakamura, and S. Kawata "3-D Microscope Imaging By Optical 3-D CT", Proc. SPIE 0813, Optics and the Information Age, (1 January 1987); https://doi.org/10.1117/12.967291

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available