23 December 2011 Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials
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Abstract
Hybrid nanoporous MCF, SBA-15 and MCM-41 materials were synthesized via hydrothermal treatment and functionalized with 3-aminopropyltriethoxysilane (APTES) via post-synthesis grafting and sequently activated by glutardialdehyde and then were used to immobilize D-amino acid oxidase (DAAO). The amino-functionalized materials were characterized by various techniques: XRD, IR and N2 adsorption-desorption (BET). From characterization results, it indicated that these materials still maintained their structure after functionalization. The data IR and TGA-DTA analysis demonstrated the incorpotation of amine functional groups on the surface of APTES-functionalized samples. The DAAO immobilized on functionalized materials exhibited higher catalytic activity and stability for conversion of cephalosporin C (CPC) compare to those of non-functionalized one. Further more, the catalytic activity as well as stability of enzyme decreased in order MCF > SBA-15 > MCM-41 with the decrease of their pore size.
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Tien Q. Phi, Tien Q. Phi, Hy G. Le, Hy G. Le, Tuan A. Vu, Tuan A. Vu, Thao T. H. Phan, Thao T. H. Phan, Huyen T. Pham, Huyen T. Pham, Canh Duc Dao, Canh Duc Dao, Phuong T. Dang, Phuong T. Dang, } "Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials", Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043I (23 December 2011); doi: 10.1117/12.903739; https://doi.org/10.1117/12.903739
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