9 February 2012 Multichannel optical brain imaging to separate cerebral vascular, tissue metabolic, and neuronal effects of cocaine
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Characterization of cerebral hemodynamic and oxygenation metabolic changes, as well neuronal function is of great importance to study of brain functions and the relevant brain disorders such as drug addiction. Compared with other neuroimaging modalities, optical imaging techniques have the potential for high spatiotemporal resolution and dissection of the changes in cerebral blood flow (CBF), blood volume (CBV), and hemoglobing oxygenation and intracellular Ca ([Ca2+]i), which serves as markers of vascular function, tissue metabolism and neuronal activity, respectively. Recently, we developed a multiwavelength imaging system and integrated it into a surgical microscope. Three LEDs of λ1=530nm, λ2=570nm and λ3=630nm were used for exciting [Ca2+]i fluorescence labeled by Rhod2 (AM) and sensitizing total hemoglobin (i.e., CBV), and deoxygenated-hemoglobin, whereas one LD of λ1=830nm was used for laser speckle imaging to form a CBF mapping of the brain. These light sources were time-sharing for illumination on the brain and synchronized with the exposure of CCD camera for multichannel images of the brain. Our animal studies indicated that this optical approach enabled simultaneous mapping of cocaine-induced changes in CBF, CBV and oxygenated- and deoxygenated hemoglobin as well as [Ca2+]i in the cortical brain. Its high spatiotemporal resolution (30μm, 10Hz) and large field of view (4x5 mm2) are advanced as a neuroimaging tool for brain functional study.
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Hugang Ren, Hugang Ren, Zhongchi Luo, Zhongchi Luo, Zhijia Yuan, Zhijia Yuan, Yingtian Pan, Yingtian Pan, Congwu Du, Congwu Du, } "Multichannel optical brain imaging to separate cerebral vascular, tissue metabolic, and neuronal effects of cocaine", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 820755 (9 February 2012); doi: 10.1117/12.910160; https://doi.org/10.1117/12.910160

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