Shining new light on optogenetics

Ling Gu; Kamal Dhakal; Ting Li; Samarendra Mohanty

doi:10.1117/12.916750

17 February 2012 Shining new light on optogenetics

Ling Gu, Kamal Dhakal, Ting Li, Samarendra Mohanty

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 820764 (2012) https://doi.org/10.1117/12.916750
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Since development of optogenetic stimulation paradigm, there has been several attempts to red shift the excitation maximum of the efficient blue-sensitive opsins. While there has been some success at the cost of altered light-activation kinetics, near-infrared optogenetic probe will be ideal for in-depth cell-specific stimulation of excitable cells in an organ. However, single-photon near-infrared optogenetics based stimulation will still limit precise probing and modulation of in-vivo neural circuits. In contrast, by virtue of non-linear nature of ultrafast light-matter interaction, high spatial precision in optogenetic activation can be achieved in addition to inherent cellular specificity and temporal resolution provided by the opsins. Here, we report use of non-linear optogenetics for stimulation of neurons in-vivo in mouse models. Advantage of using non-linear optogenetics for probing neuronal circuitry is discussed. Further, effectiveness of the non-diffracting optogenetic Bessel beam over classical Gaussian beam in a layered mouse-brain geometry is demonstrated using Monte Carlo (MC) simulation. This is corroborated by electrophysiological measurements in in-vivo mouse models. The large propagation distance, characteristics of Bessel beam is better suited for in-depth single as well as two-photon optogenetic stimulation.

Citation Download Citation

Ling Gu, Kamal Dhakal, Ting Li, and Samarendra Mohanty "Shining new light on optogenetics", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 820764 (17 February 2012); https://doi.org/10.1117/12.916750

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