9 March 2012 Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatment
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Abstract
Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20% TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED) array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged light intensity on the wound surface was 3.3 mW/cm2, the corresponding total light dose being 5.9 J/cm2. One week after injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment. In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound sepsis.
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Hiroyuki Hasegawa, Hiroyuki Hasegawa, Shunichi Sato, Shunichi Sato, Satoko Kawauchi, Satoko Kawauchi, Daizoh Saitoh, Daizoh Saitoh, Nariyoshi Shinomiya, Nariyoshi Shinomiya, Hiroshi Ashida, Hiroshi Ashida, Mitsuhiro Terakawa, Mitsuhiro Terakawa, } "Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatment", Proc. SPIE 8210, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI, 82100V (9 March 2012); doi: 10.1117/12.907643; https://doi.org/10.1117/12.907643
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