9 March 2012 Optimization of parameters in photodynamic therapy to kill p. aeruginosa with 809-nm diode laser and indocyanine green
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Abstract
The emergence of antibiotic resistant bacteria causes significant increase in deaths due to infections around the world. Nowadays, it could be impossible to find appropriate antibiotics to treat some bacterial strains, especially multidrug resistant types. Therefore, there is an urgent need to develop new and safe treatment techniques for multidrug resistant bacteria associated morbidity and mortality. In this study, Photodynamic Therapy was used to destroy these kinds of bacteria with near infrared light and Indocyanine Green. Different wavelengths of lasers mostly in the visible spectrum have been investigated for Photodynamic Therapy; however near infrared lasers have been used in very few studies. The main motivation to test photodynamic therapy with near infrared light and indocyanine green is that the near infrared laser (around 800-nm) has more penetration depth in the biological tissue than the other lasers have. Therefore it is supposed that it will show more antibacterial effect. And also indocyanine green has a very low toxicity and an FDAapproved drug. This study investigated optimum parameters for PDT with 809-nm laser and Indocyanine green (ICG) to kill P. aeruginosa in vitro. We were able to optimize the laser power and ICG concentration to non-toxic levels and achieved 99% decrease in bacterial load with 252 J/cm2 laser light and 125 μg/ml ICG concentration. This study demonstrates that PDT with near-infrared light and ICG can be powerful and non-hazardous treatment strategy for untreatable pathogens.
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Nermin Topaloglu, Nermin Topaloglu, Sahru Yuksel, Sahru Yuksel, Murat Gulsoy, Murat Gulsoy, } "Optimization of parameters in photodynamic therapy to kill p. aeruginosa with 809-nm diode laser and indocyanine green", Proc. SPIE 8210, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI, 821012 (9 March 2012); doi: 10.1117/12.910891; https://doi.org/10.1117/12.910891
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