23 February 2012 Experimental demonstration of application of ring down measurement approach to microcavities for biosensing
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Abstract
Cavity ring down measurement approach is a promising technique for biosensing as it is insensitive to intensity uctuations of a laser source. This technique in conjunction with ultra high Q microcavities have a great potential for ultra sensitive biosensing. Until now, most work on microcavity biosensors has been based on measurement of the resonant frequency shift induced by binding event on surface of the microcavity. Such measurements suer from the noise due to intensity uctuations of the laser source. However, the binding event will also introduce shift in quality factor of the microcavity, which can be tracked by using cavity ring down spectroscopy. In this work, we report on experimental demonstration of application of ring down measurement approach to microcavities for biosensing by tracking disassociation phase of a biotin-streptavidin reaction. These measurements were performed by using a bioconjugated ultra high Q microtoroidal cavity immersed in a liquid microacquarium. We found that disassociation curves agree with previously reported results on the protein kinetics measurements.
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M. Imran Cheema, Simin Mehrabani, Ahmad A. Hayat, Francis Vanier, Yves-Alain Peter, Andrea M. Armani, Andrew G. Kirk, "Experimental demonstration of application of ring down measurement approach to microcavities for biosensing", Proc. SPIE 8212, Frontiers in Biological Detection: From Nanosensors to Systems IV, 82120B (23 February 2012); doi: 10.1117/12.907422; https://doi.org/10.1117/12.907422
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