28 February 2012 Photoacoustic detection of induced melanoma in vitro using a mouse model
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Metastasis is a life threatening complex physiological phenomenon that involves the movement of cancer cells from one organ to another by means of blood and lymph. An understanding about metastasis is extremely important to device diagnostic systems to detect and monitor its spread within the body. For the first time we report rapid photoacoustic detection of the induced metastatic melanoma in mice in vitro using photoacoustic flowmetry. A new photoacoustic flow system is developed, that employs photoacoustic excitation coupled with an ultrasound transducer capable of determining the presence of individual, induced mouse melanoma cells (B16/F10) within the circulating system in vitro. Tumor was induced in mice by injecting mouse melanoma cells through tail vein into the C57BL/6 mice. A luciferase based in vivo bioluminescence imaging is performed to confirm the tumor load and multiple metastases in the tumor-induced mice. 1ml of blood obtained through cardiac puncture of the induced metastasized mice was treated to lyse the red blood cells (RBC) and enriched, leaving the induced melanoma in the peripheral blood mononuclear suspension (PBMC). A photoacoustic flowsystem coupled with an ultrasound transducer is used to detect the individual circulating metastatic melanoma cells from the enriched cell suspension.
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Sagar Gupta, Kiran Bhattacharya, Jessica R. Newton, Thomas P. Quinn, John A. Viator, "Photoacoustic detection of induced melanoma in vitro using a mouse model", Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 821414 (28 February 2012); doi: 10.1117/12.909352; https://doi.org/10.1117/12.909352

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