High-throughput Raman and surface-enhanced Raman microscopy

Ji Qi; Pratik Motwani; John C. Wolfe; Wei-Chuan Shih

doi:10.1117/12.908971

1 February 2012 High-throughput Raman and surface-enhanced Raman microscopy

Ji Qi, Pratik Motwani, John C. Wolfe, Wei-Chuan Shih

Proceedings Volume 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry; 821903 (2012) https://doi.org/10.1117/12.908971
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Line-scan Raman microscopy (LSRM) is a versatile technique for high throughput label-free chemical mapping. The LSRM instrument achieves a hundred-fold throughput advantage over conventional point-scan Raman microscopy, by projecting a laser line onto the sample and image the Raman scattered light from the entire line using a grating spectrograph and a CCD camera. Two-dimensional chemical maps can be generated by scanning the projected line in the transverse direction. Areas of 100 x 100 μm² can be rapidly mapped with sub-micron spatial resolution and 100% fill factor. The instrument enables rapid classification of microparticles with similar shape, size and refractive index based on their chemical composition. We have achieved an equivalent imaging throughput of 100 microparticles/sec for 1 μm polystyrene beads. We have extended the technology to surface-enhanced Raman imaging, by characterizing the spatial uniformity of the SERS response of several types of nanostructured plasmonic substrates.

Citation Download Citation

Ji Qi, Pratik Motwani, John C. Wolfe, and Wei-Chuan Shih "High-throughput Raman and surface-enhanced Raman microscopy", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 821903 (1 February 2012); https://doi.org/10.1117/12.908971

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available