31 January 2012 Biochemical differentiation of mycelium and yeast forms of Paracoccidioides brasiliensis by Fourier transform infrared spectroscopy
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Abstract
Paracoccidioides brasiliensis the etiological agent of paracoccidioidomycosis, is a dimorphic fungus existing as mycelia in the environment (or at 25 °C in vitro) and as yeast cells in the human host (or at 37°C in vitro). The most prominent difference between both forms is probably the cell wall polysaccharide, being 1,3-β-glucan usually found in mycelia and 1,3-α-glucan found in yeasts, but a plethora of other differences have already been described. In this work, we performed a Fourier Transform Infrared Spectroscopy analysis to compare the yeast and mycelia forms of P. brasiliensis and found additional biochemical differences. The analysis of the spectra showed that differences were distributed in chemical bonds of proteins, lipids and carbohydrates.
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Maiara L. Castilho, Claudia B. L. Campos, Tatiana G. F. Matos, Geraldo M. A. de Abreu, Airton A. Martin, Leandro Raniero, "Biochemical differentiation of mycelium and yeast forms of Paracoccidioides brasiliensis by Fourier transform infrared spectroscopy", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 821911 (31 January 2012); doi: 10.1117/12.907120; https://doi.org/10.1117/12.907120
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