31 January 2012 Buccal microbiology analyzed by infrared spectroscopy
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Abstract
Rapid microbiological identification and characterization are very important in dentistry and medicine. In addition to dental diseases, pathogens are directly linked to cases of endocarditis, premature delivery, low birth weight, and loss of organ transplants. Fourier Transform Infrared Spectroscopy (FTIR) was used to analyze oral pathogens Aggregatibacter actinomycetemcomitans ATCC 29523, Aggregatibacter actinomycetemcomitans-JP2, and Aggregatibacter actinomycetemcomitans which was clinically isolated from the human blood-CI. Significant spectra differences were found among each organism allowing the identification and characterization of each bacterial species. Vibrational modes in the regions of 3500-2800 cm-1, the 1484-1420 cm-1, and 1000-750 cm-1 were used in this differentiation. The identification and classification of each strain were performed by cluster analysis achieving 100% separation of strains. This study demonstrated that FTIR can be used to decrease the identification time, compared to the traditional methods, of fastidious buccal microorganisms associated with the etiology of the manifestation of periodontitis.
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Geraldo Magno Alves de Abreu, Geraldo Magno Alves de Abreu, Gislene Rodrigues da Silva, Gislene Rodrigues da Silva, Sônia Khouri, Sônia Khouri, Priscila Pereira Favero, Priscila Pereira Favero, Leandro Raniero, Leandro Raniero, Airton Abrahão Martin, Airton Abrahão Martin, "Buccal microbiology analyzed by infrared spectroscopy", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 821912 (31 January 2012); doi: 10.1117/12.907398; https://doi.org/10.1117/12.907398
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