23 February 2012 Optoacoustic 3D visualization of changes in physiological properties of mouse tissues from live to postmortem
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Using the method of 3D optoacoustic tomography, we studied changes in tissues of the whole body of nude mice as the changes manifested themselves from live to postmortem. The studies provided the necessary baseline for optoacoustic imaging of necrotizing tissue, acute and chronic hypoxia, and reperfusion. They also establish a new optoacoustic model of early postmortem conditions of the whole mouse body. Animals were scanned in a 37°C water bath using a three-dimensional optoacoustic tomography system previously shown to provide high contrast maps of vasculature and organs based on changes in the optical absorbance. The scans were performed right before, 5 minutes after, 2 hours and 1 day after a lethal injection of KCl. The near-infrared laser wavelength of 765 nm was used to evaluate physiological features of postmortem changes. Our data showed that optoacoustic imaging is well suited for visualization of both live and postmortem tissues. The images revealed changes of optical properties in mouse organs and tissues. Specifically, we observed improvements in contrast of the vascular network and organs after the death of the animal. We associated these with reduced optical scattering, loss of motion artifacts, and blood coagulation.
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Richard Su, Sergey A. Ermiliov, Anton V. Liopo, Alexander A. Oraevsky, "Optoacoustic 3D visualization of changes in physiological properties of mouse tissues from live to postmortem", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82230K (23 February 2012); doi: 10.1117/12.910975; https://doi.org/10.1117/12.910975

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