Hyperspectral vibrational photoacoustic imaging of lipids and collagen

Pu Wang; Ping Wang; Han-Wei Wang; Ji-Xin Cheng

doi:10.1117/12.908714

23 February 2012 Hyperspectral vibrational photoacoustic imaging of lipids and collagen

Pu Wang, Ping Wang, Han-Wei Wang, Ji-Xin Cheng

Proceedings Volume 8223, Photons Plus Ultrasound: Imaging and Sensing 2012; 82231I (2012) https://doi.org/10.1117/12.908714
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The recently developed vibrational photoacoustic (VPA) microscopy allows bond-selective imaging of deep tissues by taking advantage of intrinsic contrast from harmonic vibration of C-H bonds. Due to the spectral similarity of molecules in the overtone vibration region, the compositional information is not available from VPA images acquired by single wavelength excitation. Here we demonstrate that lipids and collagen, two critical markers in many kinds of diseases, can be distinguished by hyperspectral VPA imaging. A phantom consisted of rat tail tendon (collagen) and fat tissue (lipids) was constructed. Wavelengths between 1650 and 1850 nm were scanned to excite the first overtone/combination vibration of C-H bond. B-scan hyperspectral VPA images, in which each pixel contains a spectrum, was analyzed by a Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) algorism to recover the spatial distribution of two chemical components in the phantom.

Citation Download Citation

Pu Wang, Ping Wang, Han-Wei Wang, and Ji-Xin Cheng "Hyperspectral vibrational photoacoustic imaging of lipids and collagen", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82231I (23 February 2012); https://doi.org/10.1117/12.908714

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available