23 February 2012 In vivo imaging of cell nuclei by photoacoustic microscopy without staining
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Abstract
Ultraviolet photoacoustic microscopy (UVPAM) can image cell nuclei in vivo with high contrast and resolution noninvasively without staining. Here, we used UV light at wavelengths of 210-310 nm for excitation of DNA and RNA to produce photoacoustic waves. We applied the UVPAM to in vivo imaging of cell nuclei in mouse skin, and obtained UVPAM images of the unstained cell nuclei at wavelengths of 245-282 nm as ultrasound gel was used for acoustic coupling. The largest ratio of contrast to noise was found for the images of cell nuclei at a 250 nm wavelength.
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Da-Kang Yao, Da-Kang Yao, Ruimin Chen, Ruimin Chen, Konstantin Maslov, Konstantin Maslov, Qifa Zhou, Qifa Zhou, Lihong V. Wang, Lihong V. Wang, } "In vivo imaging of cell nuclei by photoacoustic microscopy without staining", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82231X (23 February 2012); doi: 10.1117/12.906211; https://doi.org/10.1117/12.906211
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