23 February 2012 Photoacoustic microscopy of myocardial sheet architecture in unfixed and unstained mammalian hearts
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The laminar myocardial sheet architecture and its dynamic change play a key role in myocardial wall thickening. Histology, confocal optical microscopy (COM), and diffusion tensor MRI (DTI) have been used to unveil the structures and functions of the myocardial sheets. However, histology and COM require fixation, sectioning, and staining processes, which dehydrate and deform the sheet architecture. Although DTI can delineate sheet architecture nondestructively in viable hearts, it cannot provide cellular-level resolution. Here we show that photoacoustic microscopy (PAM), with high resolution (~1 μm) and label-free detection, is appropriate for imaging 3D myocardial architecture. Perfused half-split mouse hearts were also imaged by PAM in vitro without fixation, dehydration, nor staining. The laminar myocardial sheet architecture was clearly visualized within a 0.15 mm depth range. Two populations of oppositely signed sheet angles were observed. Therefore, PAM promises to access dynamic changes of myocardial architectures in ex vivo perfused-viable hearts.
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Chi Zhang, Chi Zhang, Ya-Jian Cheng, Ya-Jian Cheng, Da-Kang Yao, Da-Kang Yao, Samuel Wickline, Samuel Wickline, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic microscopy of myocardial sheet architecture in unfixed and unstained mammalian hearts", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82232G (23 February 2012); doi: 10.1117/12.906208; https://doi.org/10.1117/12.906208

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