14 February 2012 Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging
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Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240V (2012) https://doi.org/10.1117/12.907916
Event: SPIE BiOS, 2012, San Francisco, California, United States
Upconversion nanoparticles (UCNPs) as a new kind of biological luminescence materials have many advantages comparing with organic fluorescence probes and semi-conductive quantum dots, such as sharp fluorescence emission, long emission lifetimes, high optical and chemical stability and low toxicity, especially low auto-fluorescence background and deep tissue penetration under near-infrared (NIR) light excitation for bioimaging. Herein, we demonstrate a facile approach to transfer UCNPs from hydrophobic to hydrophilic and the use of these UCNPs for cell imaging. Oleic acid-capped UCNPs based on NaYF4 were synthesized and modified with amphiphilic chitosan derivative through hydrophobic interaction. The as-prepared chitosan coated UCNPs with an average diameter of 35 nm were mono-dispersed in aqueous solution and possess good optical properties upon NIR light excitation. Cell viability assays indicated the low cytotoxicity and good biocompatibility of chitosan-coated UCNPs. Cell imaging results demonstrated that chitosan-coated UCNPs had great potential for bioimaing and biolabeling. Our work suggests a feasible method to modify OA-UCNPs with amphiphilic polymer and the promise of chitosan-based UCNPs for bioimaging application.
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Sisi Cui, Sisi Cui, Hongyan Zhu, Hongyan Zhu, Haiyan Chen, Haiyan Chen, Junmei Tian, Junmei Tian, Wei R. Chen, Wei R. Chen, Yueqing Gu, Yueqing Gu, } "Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240V (14 February 2012); doi: 10.1117/12.907916; https://doi.org/10.1117/12.907916

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