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13 February 2012 Realization of an endoscope equipped with microprojection system for optogenetics
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Optogenetics is the science where recent progresses in the field of photonics are combined with the techniques in molecular genetics to develop a methodology for modulation of neural activities.1-9 Despite enormous enthusiasm in using optogenetics for brain studies, little has been done on the engineering side such as technology development for light delivery or realization of reliable systems for optical monitoring of the induced activities. In this project, we have implemented a Digital Micromirror Device based microprojection system capable of delivering illumination patterns through a high-resolution imaging fiber bundle that guides the pattern to the region of interest on the surface or within the brain tissue. The system is also equipped with an imaging path for detection of calcium signals and monitoring the induced patterns of cellular activities. A very interesting application of the system is extracting topographic computational maps of cortex or cellular receptive fields in-vivo. It is known that such maps are the engine of information processing in the cortex. Better understanding of the structure of such maps will help to unravel the mysteries of brain higher level computations. Another application of this system is related to the high-resolution stimulation patterns that cannot be produced with electrode arrays. Production of high-resolution patterns is important in the study of specific modes of brain activities. We report the details of our optical design, preliminary results produced by testing the system on tissue, and we discuss our strategy to extract new data from the brain tissue.
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Ryan Baumgartner, Ryan Falk, and Ramin Pashaie "Realization of an endoscope equipped with microprojection system for optogenetics", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251K (13 February 2012);

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