9 February 2012 Single optical fiber probe for optogenetics
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Abstract
With the advent of optogenetics, all optical control and visualization of the activity of specific cell types is possible. We have developed a fiber optic based probe to control/visualize neuronal activity deep in the brain of awake behaving animals. In this design a thin multimode optical fiber serves as the head of the probe to be inserted into the brain. This fiber is used to deliver excitation/stimulation optical pulses and guide a sample of the emission signal back to a detector. The major trade off in the design of such a system is to decrease the size of the fiber and intensity of input light to minimize physical damage and to avoid photobleaching/phototoxicity but to keep the S/N reasonably high. Here the excitation light, and the associated emission signal, are frequency modulated. Then the output of the detector is passed through a time-lens which compresses the distributed energy of the emission signal and maximizes the instantaneous S/N. By measuring the statistics of the noise, the structure of the time lens can be designed to achieve the global optimum of S/N. Theoretically, the temporal resolution of the system is only limited by the time lens diffraction limit. By adding a second detector, we eliminated the effect of input light fluctuations, imperfection of the optical filters, and back-reflection of the excitation light. We have also designed fibers and micro mechanical assemblies for distributed delivery and detection of light.
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Ryan Falk, Ryan Falk, Mohammad Habibi, Mohammad Habibi, Ramin Pashaie, Ramin Pashaie, } "Single optical fiber probe for optogenetics", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251Z (9 February 2012); doi: 10.1117/12.907453; https://doi.org/10.1117/12.907453
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