9 February 2012 Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization
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Abstract
The development of rapid volumetric imaging systems for functional multiphoton microscopy is essential for dynamical imaging of large-scale neuronal network activity. Here, we introduce a line-illuminating temporal-focusing microscope capable of rapid three-dimensional imaging at 10-20 volumes/sec, and study the system's characteristics both theoretically and experimentally. We demonstrate that our system is capable of functional volumetric calcium imaging of distributed neuronal activity patterns, and introduce a computational strategy for activity reconstruction in strongly scattering media.
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Hod Dana, Hod Dana, Anat Marom, Anat Marom, Nimrod Kruger, Nimrod Kruger, Aviv Ellman, Aviv Ellman, Shy Shoham, Shy Shoham, } "Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822603 (9 February 2012); doi: 10.1117/12.908844; https://doi.org/10.1117/12.908844
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