Fluorescence lifetime imaging microscopy (FLIM) studies of living primary human cells for applications in tissue regeneration

William R. Lloyd; Leng-Chun Chen; Shiuhyang Kuo; Cynthia L. Marcelo; Stephen E. Feinberg; Mary-Ann Mycek

doi:10.1117/12.910790

9 February 2012 Fluorescence lifetime imaging microscopy (FLIM) studies of living primary human cells for applications in tissue regeneration

William R. Lloyd, Leng-Chun Chen, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, Mary-Ann Mycek

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82260E (2012) https://doi.org/10.1117/12.910790
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) was employed to noninvasively characterize metabolic function in primary human oral keratinocytes used to develop functional engineered tissues. Living cells were compared under control culture conditions and systematic variations to investigate cellular function and viability. Nonlinear optical microscopy via two-photon excitation was employed to image cellular metabolic biomarkers NAD(P)H and FAD with thin optical sectioning and minimal out-of-focus fluorophore photobleaching. Novel post-processing FLIM algorithms were developed and tested. Results suggest that FLIM may provide useful information about live cell function and viability.

Citation Download Citation

William R. Lloyd, Leng-Chun Chen, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, and Mary-Ann Mycek "Fluorescence lifetime imaging microscopy (FLIM) studies of living primary human cells for applications in tissue regeneration", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260E (9 February 2012); https://doi.org/10.1117/12.910790

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