15 February 2012 Watching stem cells at work with a flexible multiphoton tomograph
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Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82260V (2012); doi: 10.1117/12.908027
Event: SPIE BiOS, 2012, San Francisco, California, United States
Abstract
There is a high demand for non-invasive imaging techniques that allow observation of stem cells in their native environment without significant input on cell metabolism, reproduction, and behavior. Easy accessible hair follicle pluripotent stem cells in the bulge area and dermal papilla are potential sources for stem cell based therapy. It has been shown that these cells are able to generate hair, non-follicle skin cells, nerves, vessels, smooth muscles etc. and may participate in wound healing processes. We report on the finding of nestin-GFP expressing stem cells in their native niche in the bulge of the hair follicle of living mice by using high-resolution in-vivo multiphoton tomography. The 3D imaging with submicron resolution was based on two-photon induced fluorescence and second harmonic generation (SHG) of collagen. Migrating stem cells from the bulge to their microenvironment have been detected inside the skin during optical deep tissue sectioning.
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Aisada Uchugonova, Robert Hoffmann, Martin Weinigel, Karsten König, "Watching stem cells at work with a flexible multiphoton tomograph", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260V (15 February 2012); doi: 10.1117/12.908027; https://doi.org/10.1117/12.908027
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KEYWORDS
Stem cells

Skin

Tissue optics

In vivo imaging

Second-harmonic generation

Green fluorescent protein

Image resolution

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