15 February 2012 Watching stem cells at work with a flexible multiphoton tomograph
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Abstract
There is a high demand for non-invasive imaging techniques that allow observation of stem cells in their native environment without significant input on cell metabolism, reproduction, and behavior. Easy accessible hair follicle pluripotent stem cells in the bulge area and dermal papilla are potential sources for stem cell based therapy. It has been shown that these cells are able to generate hair, non-follicle skin cells, nerves, vessels, smooth muscles etc. and may participate in wound healing processes. We report on the finding of nestin-GFP expressing stem cells in their native niche in the bulge of the hair follicle of living mice by using high-resolution in-vivo multiphoton tomography. The 3D imaging with submicron resolution was based on two-photon induced fluorescence and second harmonic generation (SHG) of collagen. Migrating stem cells from the bulge to their microenvironment have been detected inside the skin during optical deep tissue sectioning.
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Aisada Uchugonova, Aisada Uchugonova, Robert Hoffmann, Robert Hoffmann, Martin Weinigel, Martin Weinigel, Karsten König, Karsten König, } "Watching stem cells at work with a flexible multiphoton tomograph", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260V (15 February 2012); doi: 10.1117/12.908027; https://doi.org/10.1117/12.908027
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