9 February 2012 High-speed spectral tuning CARS microscopy using AOTF laser
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Abstract
We have developed a high speed spectral tuning CARS microscopy system using a mode-locked Ti:Sapphire laser with an acousto-optic tunable filter (AOTF) in the cavity. Since the wavelength of the laser is tunable with the applied radio frequency to the AOTF, the wavelength is electrically tunable.The pulse duration of the laser is about 10 ps, tunable range is 800 nm to 930 nm, and the tuning speed is ms order. The laser is synchronized with another mode-locked Ti:Sapphire laser laser our own method using a balance cross-correlator and phase lock loop technique. The synchronized lasers are used for light source of multi-focus CARS microscopy system using a microlens array scanner, and the hyperspectral imaging of adipocyte cells is demonstrated.
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Mamoru Hashimoto, Mamoru Hashimoto, Junichi Iwatsuka, Junichi Iwatsuka, Hirohiko Niioka, Hirohiko Niioka, Tsutomu Araki, Tsutomu Araki, } "High-speed spectral tuning CARS microscopy using AOTF laser", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261N (9 February 2012); doi: 10.1117/12.909507; https://doi.org/10.1117/12.909507
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