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9 February 2012 A novel multimodal CARS miniaturized microscope
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Abstract
We demonstrate the operation of a novel portable, fibre delivery miniaturized multimodal microscope (exoscope) for coherent anti-Stokes Raman scattering and two-photon excitation fluorescence imaging using a single Ti:sapphire femtosecond pulsed laser. This microscope features a large mode area photonic crystal fibre for light delivery, as well as biaxial scanning microelectromechanical system mirrors and custom miniaturized optics corrected for chromatic aberration. We demonstrate imaging of polystyrene beads, two photon excitation fluorescence beads in both forward and backward (epi) directions. This miniaturized exoscope will enable in-vivo imaging of rat spinal cord.
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Brett Smith, Majid Naji, Sangeeta Murugkar, Craig Brideau, Peter Stys, and Hanan Anis "A novel multimodal CARS miniaturized microscope", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822629 (9 February 2012); https://doi.org/10.1117/12.909494
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