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9 February 2012 Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system
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Confocal microscopy can be used as a practical tool in non-invasive applications in medical diagnostics and evaluation. In particular, it is being used for the early detection of skin cancer to identify pathological cellular components and, potentially, replace conventional biopsies. The detection of melanin and its spatial location and distribution plays a crucial role in the detection and evaluation of skin cancer. Our previous work has shown that the visible emission from melanin is strong and can be easily observed with a near-infrared CW laser using low power. This is due to a unique step-wise, (SW) three-photon excitation of melanin. This paper shows that the same SW, 3-photon fluorescence can also be achieved with an inexpensive, continuous-wave laser using a dual-prism scanning system. This demonstrates that the technology could be integrated into a portable confocal microscope for clinical applications. The results presented here are in agreement with images obtained with the larger and more expensive femtosecond laser system used earlier.
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Yair Mega, Joseph Kerimo, Joseph Robinson, Ali Vakili, Nicolette Johnson, and Charles DiMarzio "Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822637 (9 February 2012);

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