Correction accuracy in image-based adaptive optics for nonlinear microscopy

Aurélie Facomprez; Emmanuel Beaurepaire; Delphine Débarre

doi:10.1117/12.908647

2 February 2012 Correction accuracy in image-based adaptive optics for nonlinear microscopy

Aurélie Facomprez, Emmanuel Beaurepaire, Delphine Débarre

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Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 822709 (2012) https://doi.org/10.1117/12.908647
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.

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Aurélie Facomprez, Emmanuel Beaurepaire, and Delphine Débarre "Correction accuracy in image-based adaptive optics for nonlinear microscopy", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 822709 (2 February 2012); https://doi.org/10.1117/12.908647

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KEYWORDS

Photons

Aberration correction

Adaptive optics

Microscopy

Sensors

Monochromatic aberrations

Evolutionary algorithms

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