8 February 2012 Melanin fluorescence spectra by step-wise three photon excitation
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Melanin is the characteristic chromophore of human skin with various potential biological functions. Kerimo discovered enhanced melanin fluorescence by stepwise three-photon excitation in 2011. In this article, step-wise three-photon excited fluorescence (STPEF) spectrum between 450 nm -700 nm of melanin is reported. The melanin STPEF spectrum exhibited an exponential increase with wavelength. However, there was a probability of about 33% that another kind of step-wise multi-photon excited fluorescence (SMPEF) that peaks at 525 nm, shown by previous research, could also be generated using the same process. Using an excitation source at 920 nm as opposed to 830 nm increased the potential for generating SMPEF peaks at 525 nm. The SMPEF spectrum peaks at 525 nm photo-bleached faster than STPEF spectrum.
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Zhenhua Lai, Zhenhua Lai, Josef Kerimo, Josef Kerimo, Charles A. DiMarzio, Charles A. DiMarzio, } "Melanin fluorescence spectra by step-wise three photon excitation", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270J (8 February 2012); doi: 10.1117/12.908710; https://doi.org/10.1117/12.908710

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