Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues

Christopher Glazowski; James Zavislan; Milind Rajadhyaksha

doi:10.1117/12.908270

2 February 2012 Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues

Christopher Glazowski, James Zavislan, Milind Rajadhyaksha

Author Affiliations +

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82270Z (2012) https://doi.org/10.1117/12.908270
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We present a design of a line-scanning confocal microscope for reduced speckle and improved sectioning performance by the use of two pupil-modification techniques. The first is a divided-pupil configuration in which the illumination and detection paths are separate in object space except in the focal (optical sectioning) plane. The second technique is a novel implementation of a Nomarski prism and quarter-wave retarder, termed NRDIC, which has shown good results in point-scanning confocal microscopy and we will present its translation to line-scanning confocal microscopy. A stable turbid phantom that simulates the background-driven speckle was used for quantitative characterization. Compared to standard full pupil line-scanning, we show improvements in signal to background of 1.8 and 9 for NRDIC and divided pupil, respectively. Preliminary imaging in human skin in-vivo demonstrates the improvements in contrast and reduction of speckle for both the NRDIC and divided pupil modes.

Citation Download Citation

Christopher Glazowski, James Zavislan, and Milind Rajadhyaksha "Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270Z (2 February 2012); https://doi.org/10.1117/12.908270

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