Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease

Natan T. Shaked; Lisa L. Satterwhite; Marilyn J. Telen; George A. Truskey; Adam Wax

doi:10.1117/12.907659

2 February 2012 Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease

Natan T. Shaked, Lisa L. Satterwhite, Marilyn J. Telen, George A. Truskey, Adam Wax

Author Affiliations +

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 822711 (2012) https://doi.org/10.1117/12.907659
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We have applied wide-field digital interferometric techniques to quantitatively image sickle red blood cells (RBCs) [1] in a noncontact label-free manner, and measure the nanometer-scale fluctuations in their thickness as an indication of their stiffness. The technique can simultaneously measure the fluctuations for multiple spatial points on the RBC and thus yields a map describing the stiffness of each RBC in the field of view. Using this map, the local rigidity regions of the RBC are evaluated quantitatively. Since wide-field digital interferometry is a quantitative holographic imaging technique rather than one-point measurement, it can be used to simultaneously evaluate cell transverse morphology plus thickness in addition to its stiffness profile. Using this technique, we examine the morphology and dynamics of RBCs from individuals who suffer from sickle cell disease, and find that the sickle RBCs are significantly stiffer than healthy RBCs. Furthermore, we show that the technique is sensitive enough to distinguish various classes of sickle RBCs, including sickle RBCs with visibly-normal morphology, compared to the stiffer crescent-shaped sickle RBCs.

Citation Download Citation

Natan T. Shaked, Lisa L. Satterwhite, Marilyn J. Telen, George A. Truskey, and Adam Wax "Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 822711 (2 February 2012); https://doi.org/10.1117/12.907659

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