13 February 2012 Stokes vector formalism based second harmonic generation microscopy
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Abstract
In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.
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Jianjun Qiu, Nirmal Mazumder, Han-Ruei Tsai, Chih-Wei Hu, Fu-Jen Kao, "Stokes vector formalism based second harmonic generation microscopy", Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280C (13 February 2012); doi: 10.1117/12.907199; https://doi.org/10.1117/12.907199
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