2 February 2012 Silica suspended waveguide splitter-based biosensor
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Recently, a novel integrated optical waveguide 50/50 splitter was developed. It is fabricated using standard lithographic methods, a pair of etching steps and a laser reflow step. However, unlike other integrated waveguide splitters, the waveguide is elevated off of the silicon substrate, improving its interaction with biomolecules in solution and in a flow field. Additionally, because it is fabricated from silica, it has very low optical loss, resulting in a high signal-to-noise ratio, making it ideal for biosensing. By functionalizing the device using an epoxy-silane method using small samples and confining the protein solutions to the device, we enable highly efficient detection of CREB with only 1 μL of solution. Therefore, the waveguide coupler sensor is representative of the next generation of ultra-sensitive optical biosensors, and, when combined with microfluidic capabilities, it will be an ideal candidate for a more fully-realized lab-on-a-chip device.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. C. Harrison, M. C. Harrison, R. M. Hawk, R. M. Hawk, A. M. Armani, A. M. Armani, "Silica suspended waveguide splitter-based biosensor", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290E (2 February 2012); doi: 10.1117/12.907393; https://doi.org/10.1117/12.907393

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