2 February 2012 Surface enhanced biodetection on a CMOS biosensor chip
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Abstract
We present a rigorous electromagnetic theory of the electromagnetic power emitted by a dipole located in the vicinity of a multilayer stack. We applied this formalism to a luminescent molecule attached to a CMOS photodiode surface and report light collection efficiency larger than 80% toward the CMOS silicon substrate. We applied this result to the development of a low-cost, simple, portable device based on CMOS photodiodes technology for the detection and quantification of biological targets through light detection, presenting high sensitivity, multiplex ability, and fast data processing. The key feature of our approach is to perform the analytical test directly on the CMOS sensor surface, improving dramatically the optical detection of the molecule emitted light into the high refractive index semiconductor CMOS material. Based on adequate surface chemistry modifications, probe spotting and micro-fluidics, we performed proof-of-concept bio-assays directed against typical immuno-markers (TNF-α and IFN-γ). We compared the developed CMOS chip with a commercial micro-plate reader and found similar intrinsic sensitivities in the pg/ml range.
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Federico Belloni, Federico Belloni, Laure Sandeau, Laure Sandeau, Sylvain Contié, Sylvain Contié, Florence Vicaire, Florence Vicaire, Roisin Owens, Roisin Owens, Hervé Rigneault, Hervé Rigneault, } "Surface enhanced biodetection on a CMOS biosensor chip", Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310L (2 February 2012); doi: 10.1117/12.907285; https://doi.org/10.1117/12.907285
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