2 February 2012 Multiphoton fluorescence spectra and lifetimes of biliverdins and their protein-associated complex
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To investigate whether endogenous biliverdins can serve as a fluorescence metabolic marker in cancer diagnosis, we measured their multiphoton fluorescence spectra and lifetimes with femtosecond Cr:forsterite laser. Excited at 1230nm, the two-photon fluorescence of biliverdins peaks around 670nm. The corresponding lifetime (<100ps) was much shorter than those of porphyrins (~10ns), which is another commonly present metabolites in living cells. Further mixing biliverdins with proteins like fetal bovine serum (FBS), biliverdins reductase A (BVRA), or heme oxygenase-1 (HO-1), the yields of red autofluorescences didn't change a lot, but the corresponding lifetimes with HO-1 and BSA were lengthened to 200~300ps. This indicates that biliverdin can have an association with these proteins and change its lifetime. These spectral and temporal characteristics of fluorescence make biliverdin a potential marker fluorophore for hyperspectral diagnosis on the heme catabolism in human cells or tissues.
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Chin-Jie Huang, Chin-Jie Huang, Cheng-Ham Wu, Cheng-Ham Wu, Tzu-Ming Liu, Tzu-Ming Liu, "Multiphoton fluorescence spectra and lifetimes of biliverdins and their protein-associated complex", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330P (2 February 2012); doi: 10.1117/12.907725; https://doi.org/10.1117/12.907725

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