2 February 2012 The observation research of the differences in cell death and reactive oxygen species in the process of infecting Arabidopsis with avirulent strains
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Abstract
Objective: To observe the differences of cell death and accumulation of reactive oxygen species (ROS) in the process of infecting Arabidopsis with avirulent Pseudomonas syringae pv. tomato DC3000 (avrB, avrRps4), it will be of great importance to research the role of plant disease resistance and defense response. Methods: Using WT, AtrbohD and AtrbohF mutant as experimental materials, we discuss the impact of cell death and ROS on the leaves of Arabidopsis infected with avirulent Pst DC3000 (avrB, avrRps4), observed by spectral analysis and visualized by DAB and trypan blue stain. Results: When infected with avirulent Pst DC3000, both WT and AtrbohF mutant line behaved resistance that exhibited burst of ROS and HR occur, limit senescence and pathogen induced chlorotic cell death. Paradoxically, AtrbohD mutant behaved susceptible characters that exhibited a small quantity of ROS accumulated and enhanced cell death. Conclusion: After infection of Arabidopsis with avirulent Pst DC3000, WT exhibited more ROS accumulation than AtrbohF, and AtrbohD eliminated the majority of total ROS production. Although both WT and AtrbohF mutant exhibited HR occur, enhanced cell death in AtrbohD mutant.
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HuaBin Liu, HuaBin Liu, WenLi Chen, WenLi Chen, } "The observation research of the differences in cell death and reactive oxygen species in the process of infecting Arabidopsis with avirulent strains", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331F (2 February 2012); doi: 10.1117/12.907168; https://doi.org/10.1117/12.907168
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