6 February 2012 Single cell targeting using plasmon resonant gold-coated liposomes
Author Affiliations +
Proceedings Volume 8234, Plasmonics in Biology and Medicine IX; 82341J (2012) https://doi.org/10.1117/12.909314
Event: SPIE BiOS, 2012, San Francisco, California, United States
We have developed an experimental system with the potential for the delivery and localized release of an encapsulated agent with high spatial and temporal resolution. We previously introduced liposome-supported plasmon resonant gold nanoshells; in this composite structure, the liposome allows for the encapsulation of substances, such as therapeutic agents, neurotransmitters, or growth factors, and the plasmon resonant structure facilitates the rapid release of encapsulated contents upon laser light illumination. More recently, we demonstrated that these gold-coated liposomes are capable of releasing their contents in a spectrally-controlled manner, where plasmon resonant nanoparticles only release content upon illumination with a wavelength of light matching their plasmon resonance band. We now show that this release mechanism can be used in a biological setting to deliver a peptide derivative of cholecystokinin to HEK293 cells overexpressing the CCK2 receptor. Using directed laser light, we may enable localized release from gold-coated liposomes to enable accurate perturbation of cellular functions in response to released compounds; this system may have possible applications in signaling pathways and drug discovery.
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Sarah J. Leung, Sarah J. Leung, Marek Romanowski, Marek Romanowski, } "Single cell targeting using plasmon resonant gold-coated liposomes", Proc. SPIE 8234, Plasmonics in Biology and Medicine IX, 82341J (6 February 2012); doi: 10.1117/12.909314; https://doi.org/10.1117/12.909314

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