14 February 2012 Magnetic particle-based sample-prep and valveing in microfluidic devices
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Abstract
There is a need to design an integrated microfluidic platform as simple and lean as possible in order to meet the requirements for a miniaturized system. Magnetic particles show a great versatility in performing several of the functions necessary in many microfluidic assays. We therefore have developed a compact portable system to perform magneticbead- based sample preparation steps in a chip such as DNA-extraction or particle-enhanced mixing of reagents. A central application in a standard biochemical/biological/medical laboratory is represented by PCR. The execution of a cyclic heating profile during PCR is a considerable stress for chip and liquid inside the chip because evaporation and uncontrolled condensation or unintended motion of the PCR solution. One strategy to overcome this problem consists of the implementation of valves flanking a stationary PCR in appropriate incubation cavities. In addition to the well-known elastomeric membrane valves, wax-valves mechanical turning or rotary valves flanking the PCR chamber, we present in this paper the use of clustered magnetic particles as blocking valves for such reaction chambers. We report on the capability of assembled magnetic particles to act as rather simple configurated valves during a PCR typical temperature regime. These novel valves efficiently withstand 1.5 bar pressure, prevent loss of aqueous liquid inside the reaction chamber via evaporation or bubble formation, and do not express adverse effects on any biological reaction inside the chip-based PCR cavity. The latter properties have been proven by a set of different PCRs performed in chip-based cavities.
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Richard Klemm, Richard Klemm, Nadine Hlawatsch, Nadine Hlawatsch, Thomas E. Hansen-Hagge, Thomas E. Hansen-Hagge, Holger Becker, Holger Becker, Claudia Gärtner, Claudia Gärtner, } "Magnetic particle-based sample-prep and valveing in microfluidic devices", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 825108 (14 February 2012); doi: 10.1117/12.910179; https://doi.org/10.1117/12.910179
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