14 February 2012 Spiral inertial microfluidic devices for continuous blood cell separation
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Abstract
Enrichment and separation of cell components of blood is critical to clinical diagnostics and therapeutics. Here we report on spiral inertial microfluidic devices which achieve continuous size-based separation of cell mixtures with high throughput. These devices rely on hydrodynamic forces acting on cells within laminar flow, coupled with Dean instability-induced drag arising from the spiral microchannel geometry, to focus cells in streams near the inner channel wall. The spiral devices were optimized to achieve cell separation in less than 8 cm. These improved devices represent an important development because they are not only small in size (<1 in2), but exhibit high separation efficiency (~90%) and high throughput rates up to 1 million cells per minute. These device concepts offer a path towards possible development of a lab-on-chip for blood analysis and reagent free sample preparation, illustrated by the present results, which successfully demonstrate separation of erythrocytes from leukocytes with whole blood.
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Nivedita Nivedita, Phillip Ligrani, Ian Papautsky, "Spiral inertial microfluidic devices for continuous blood cell separation", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510R (14 February 2012); doi: 10.1117/12.909936; https://doi.org/10.1117/12.909936
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