14 February 2012 Multiplexed fluorescence detection for point of care
Author Affiliations +
Abstract
We report on the development of a portable fluorescence detection system. By combining a CMOS sensor and crosspolarization scheme, we achieved multiplexed detection with a single white emission LED excitation. We demonstrated fluorescence detection of Fluorescein and Rhodamine B in PDMS channels and achieved 1μM limit of detection (LOD). Microparticles with green and red fluorescence were detected simultaneously without changing the light sources or filters. We were able to clearly resolve microparticles, even if aggregated. The compact microfluorescence approach offers high spatial and spectral resolution, and is suitable for multiplexed detection in point-of-care applications.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Li Shen, Li Shen, Mike Ratterman, Mike Ratterman, Tyler Stites, Tyler Stites, David Klotzkin, David Klotzkin, Ian Papautsky, Ian Papautsky, "Multiplexed fluorescence detection for point of care", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510S (14 February 2012); doi: 10.1117/12.910061; https://doi.org/10.1117/12.910061
PROCEEDINGS
7 PAGES


SHARE
Back to Top