Adaptive optics for biological microscopy using phase diversity

Peter Kner

doi:10.1117/12.909241

15 February 2012 Adaptive optics for biological microscopy using phase diversity

Peter Kner

Proceedings Volume 8253, MEMS Adaptive Optics VI; 82530G (2012) https://doi.org/10.1117/12.909241
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

Phase Diversity is a powerful technique for estimating wavefront aberrations from images of extended scenes. Phase Diversity was developed for two-dimensional imaging, typically using defocus as the phase diverse aberration. Here we discuss different approaches for extending phase diversity to three-dimensional imaging for biological applications. We show the results of using phase diversity to determine wavefront aberrations on simulated images.

Citation Download Citation

Peter Kner "Adaptive optics for biological microscopy using phase diversity", Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530G (15 February 2012); https://doi.org/10.1117/12.909241

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