Development of a compact bio-optofluidic cell sorter

Andrew Banas; Darwin Palima; Finn Pedersen; Jesper Glückstad

doi:10.1117/12.909908

10 February 2012 Development of a compact bio-optofluidic cell sorter

Andrew Banas; Darwin Palima; Finn Pedersen; Jesper Glückstad

Author Affiliations +

Proceedings Volume 8274, Complex Light and Optical Forces VI; 82740N (2012) https://doi.org/10.1117/12.909908
Event: SPIE OPTO, 2012, San Francisco, California, United States

Abstract

We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

Citation Download Citation

Andrew Banas, Andrew Banas, Darwin Palima, Darwin Palima, Finn Pedersen, Finn Pedersen, Jesper Glückstad, Jesper Glückstad, } "Development of a compact bio-optofluidic cell sorter", Proc. SPIE 8274, Complex Light and Optical Forces VI, 82740N (10 February 2012); doi: 10.1117/12.909908; https://doi.org/10.1117/12.909908

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